Activity map of GPCR revealed by machine learning

Development of Intelligent Plasma Medical Technology Applying User Data Platform

AGNES medical project to prove the effectiveness of plasma laser treatment in dermatology

• Measured  reactive  oxygen  species  and  immunofluorescence staining cell  adhesion  protein (VE-cadherin, Factin, Phalloidin)

• Cultured different types of cells including HEK 293T, HELA, MCF-10A, MDA-MB-231, HACAT
• Operated cell viability assay and intracellular delivery (lipofectamine)    

MPCT (Micro Patterning Cellular Transfer)

• Designed and manufactured master mold using the photo-lithography method, and replicated patterned PDMS stamp
• Used mini prep and tested cell transfection

• Imaged optical microscopy and fluorescence microscopy and analyzed with image J       

Elastic Nanoneedle array film

SAMSUNG initiated a project to promote future technology 

• Customized nanoneedle density and shape using photocatalyst-based polymer microsurface treatment and dry etching

• Cultured and maintained HACAT, L929, HEK 293T

• Sonoporated cells with flexible nanoneedle to form nanoporosity for cell transfection (BE4max and Cas9)

• 2021 International Symposium and Annual Meeting of the KSABC, 1st Author, The Korean Society for Applied Biological Chemistry

• Publication

  Park, H.-J.; Hong, H.; Thangam, R.; Song, M.-G.; Kim, J.-E.; Jo, E.-H.; Jang, Y.-J.; Choi, W.-H.; Lee, M.-Y.; Kang, H.; Lee, K.-B. Static and Dynamic Biomaterial Engineering for Cell Modulation. Nanomaterials 2022, 12, 1377. https://doi.org/10.3390/nano12081377